Hormonally Defined Media: A Tool in Cell Biology Lectures and Posters Presented at the First European Conference on Serum-Free Cell Culture Heidelberg, October 7–9, 1982

Front Matter....Pages I-XIV
Front Matter....Pages 1-1
Divining the Role of Serum in Cell Culture....Pages 2-3
Front Matter....Pages 5-5
Reducing the Requirement for Serum Supplements in High Yield Microcarrier Cell Culture....Pages 6-15
Growth of Normal Human Cells in Defined Media....Pages 16-30
Investigations on the Cultivation of Insect Cell Lines in Serum-Free Media....Pages 31-43
Front Matter....Pages 45-45
Different Signalling Systems Control the Initiation of DNA Replication in Cultured Animal Cells....Pages 46-56
Ectopic Auto-Stimulation of Growth....Pages 57-67
The Growth and Differentiation of Human Endothelial Cells....Pages 68-78
3T3 Cell Growth Initiation and Multiplication in Defined Medium Supplemented with Growth Factors and Hormones That Replace Serum....Pages 79-87
Growth-Promoting Activity of Thrombin in Fibroblasts: Temporal Action and Early Biochemical Events in the G0 → G1 Transition....Pages 88-102
Pure Platelet-Derived Growth Factor Stimulates Human Fibroblasts to Proliferate in Plasma-Free Culture....Pages 103-106
Synergistic Effect of Retinoic Acid on DNA Synthesis of Prostaglandin F 2α Stimulated Swiss 3T3 Cells....Pages 107-110
The Secretion of Growth Regulatory Molecules by PC13 Embryonal Carcinoma Cells....Pages 111-113
Growth Regulation of L-929 Mouse Fibroblasts by Steroid Hormones and Anti-Hormones in Serum Containing and Serum Free Media....Pages 114-119
Synergistic Interactions of Specific Prostaglandins in Regulating the Rate of Initiation of DNA Synthesis in Swiss 3T3 Cells....Pages 120-122
Use of Eye Derived Growth Factor from Retina (EDGF) in a Defined Medium for the Culture of Bovine Epithelial Lens Cells....Pages 123-126
Effect of Partially Purified Preparation of Human Somatomedin A in the Three Cultured Cell Systems....Pages 127-130
Front Matter....Pages 131-131
Role of Cell-Substratum Interactions in the Hormonal Control of Rat Prolactin Cells....Pages 132-142
Control by the Extracellular Environment of Differentiation Pathways in an Embryonal Carcinoma Cell Line....Pages 143-150
Interaction of Metastatic and Non-Metastatic Tumor Lines with Aortic Endothelial Cell Monolayer and Their Underlying Basal Lamina....Pages 151-161
Control of Behaviour and Growth by Imitating the Contact Environment....Pages 162-175
Front Matter....Pages 177-177
Cells of the Peripheral Nervous System; Requirements for Expression of Function in Tissue Culture....Pages 178-188
Selection and Cultivation of Astrocytes at Different Developmental Stages....Pages 189-202
Cholinergic Differentiation in Serum-Free Aggregating Fetal Brain Cells....Pages 203-214
DNA Synthesis in Nuclei and Mitochondria Purified from Serum-Free or Supplemented Glial Cell Cultures....Pages 215-218
Guanylate Cyclase Activators Hemin and Sodium Nitroprusside Stimulate the Growth of Transformed Cells in Serum-Free Medium....Pages 219-221
Tracing the Astroglial Cell-Lineage in Vitro by Modification of the Microenvironmental Conditions....Pages 222-224
Growth of Perfused and Nonperfused Neuronal Cell Lines in Serum Deprived Medium....Pages 225-228
Primary Rat Astroglial Culture in Serum-Free Supplemented Media and Effect of a Bovine Brain Factor....Pages 229-231
Front Matter....Pages 233-233
Hormone Inducible Specific Gene Expression in an Isolated Whole Mammary Organ in Serum-Free Culture....Pages 234-249
A New Culture Technique: An Approach to the in-Vitro Reconstitution of Endocrine Organs....Pages 250-263
Hormonal Regulation of Growth and Function of Insulin-Producing Cells in Culture....Pages 264-273
Studies on Regulation of Ovarian Steroidogenesis in Vitro: The Need for a Serum-Free Medium....Pages 274-284
Control of Normal Phenotype and of Thyroid Differentiated Functions in the Epithelial Cell Strain FRTL....Pages 285-293
Butyrate-Induced Growth Arrest of GH 3 -Cells is not Linked to a Distinct Morphological Phenotype....Pages 294-297
Front Matter....Pages 299-299
Human B Cell Function in 20 μl Hanging-Drop Microcultures Under Serum-Free Conditions....Pages 300-303
Adipose Conversion of Murine Bone Marrow-Derived Macrophages in Serum-Free Medium....Pages 304-306
Analysis of the Mitogenic Effect of Fibrinogen....Pages 307-309
Hybridoma Formation in Serum-Free Medium....Pages 310-313
Immune (y) Interferon Produced by Murine T-Cell Lymphomas....Pages 314-316
TCGF-Dependent Growth of Naturally Autoreactive Cells: Regulatory Role of the Thymus....Pages 317-319
Front Matter....Pages 299-299
Serum Replacement in Culture of Human Erythroid and Megakaryocytic Precursors....Pages 320-323
Participation of Pterins and of a Pteridine Binding Variant of Alpha 1 -Acid Glycoprotein in the Control of Lymphocyte Transformation and of Lymphoblast Proliferation....Pages 324-327
Front Matter....Pages 329-329
Conversion of Heterogeneous Liver Parenchyma Cells into a Homogeneous Cell Population During Cell Culture with Respect to PEPCK....Pages 330-332
Preservation of the Adult Functionality of Hepatocytes in Serum-Free Cultures....Pages 333-336
Isolation and Characterization of MDCK-Subcultures....Pages 337-339
The Role of Fetal Calf Serum (FCS) During the First Stages of Hepatocytes Cultures....Pages 340-343
Modulation of Specific Functions in Adult and Fetal Hepatocytes Maintained in a Co-Cultured System....Pages 344-346
Cell Culture and Differentiated Properties of Nephron Epithelial Cells in Defined Medium....Pages 347-350
Multiplication of Adult Rat Hepatocytes in Monolayer Cultures....Pages 351-353
Short-Term Modulation of Glycogenolysis, Glycolysis and Gluconeogenesis in Cultured Hepatocytes by Arterial and Venous Oxygen Concentrations....Pages 354-356
Front Matter....Pages 357-357
Differentiation of Primary Muscle Cells Cultured in a Serum-Free Chemically Defined Medium....Pages 358-365
Study of a Teratoma-Derived Adipogenic Cell Line 1246 and Isolation of an Insulin-Independent Variant in Serum-Free Medium....Pages 366-376
Primary and Secondary Cultures of Rabbit Articular Chondrocytes in a Serum-Free Medium....Pages 377-379
Vanadate Antagonizes Detrimental Effects of Serum Deprivation in Cultured Rat Heart Muscle Cells....Pages 380-383
Characterization of Cardiomyocytes Cultured in Serum-Free Medium....Pages 383-386
Serum-Free Cultures of Mouse LM Fibroblasts as a Tool for Biochemical and Genetic Studies in the Cellular Responses to Interferon....Pages 387-389
Separation and Cultivation of Normal Human Melanocytes....Pages 390-392
The Use of Cytodex 3 Microcarriers and Serum-Free Media for the Production of a Nerve Growth Promoter from Chicken Heart Cells....Pages 393-395
Control of Cell Proliferation and Differentiation in the Myogenic Cell Line L6 by Manipulation of Culture Conditions....Pages 396-399
Serum-Free Culture of Adult Rat Cardiac Myocytes....Pages 400-402
Front Matter....Pages 357-357
Secretion of α-MSH-Like Molecules in a Human Melanoma Cell Line....Pages 403-406
Growth and Differentiation of Human Muscle Cells in Defined Medium....Pages 407-410
Front Matter....Pages 411-411
Tissue Culture of Human Colon Carcinomas. Therapy Experiments in Vitro....Pages 412-417
Human Prostatic Cells in Serum-Free Medium....Pages 418-429
Culture of Human Lung Carcinoma Cells in Serum-Free Media....Pages 430-435
Continuous Growth of a Human Breast Cancer Cell Line (MCF-7) in Serum-Free Medium....Pages 436-438
Regulation of Oestrogen Responsiveness of MCF-7 Human Breast Cancer Cell Growth by Serum Concentration in the Culture Medium....Pages 439-442
Induction of Differentiation in Human Solid Tumor Cells by Anti-Tumor Agents in Serum Depleted Medium....Pages 443-445
Hormonal Requirements of in Vitro Growth in Human Mammary and Ovarian Carcinoma Cell Cultures....Pages 446-448
Back Matter....Pages 449-455