Nonradioactive Labeling and Detection of Biomolecules

Front Matter....Pages I-XXIII
General Aspects of Nonradioactive Labeling and Detection....Pages 1-24
Front Matter....Pages 25-25
Overview of Nonradioactive Labeling Systems....Pages 27-34
The Digoxigenin: Anti-Digoxigenin (DIG) System....Pages 35-69
The Biotin System....Pages 70-100
In Vivo Labeling of DNA Probes with 5-BrdU....Pages 101-109
The Sulfone System....Pages 110-115
Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Probes....Pages 116-126
Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence Detection....Pages 127-134
A Highly Sensitive Method for Detecting Peroxidase in In Situ Hybridization or Immunohistochemical Assays....Pages 135-142
The SNAP System....Pages 143-149
Front Matter....Pages 151-151
Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems....Pages 153-158
Colorimetric Systems....Pages 159-164
Luminescence Systems....Pages 165-184
Fluorescence Systems....Pages 185-193
Front Matter....Pages 195-195
Overview of Amplification Systems....Pages 197-205
Enhanced Signal Generation by Target Amplification....Pages 206-239
Signal Amplification Systems: Substrate Cascade....Pages 240-243
Front Matter....Pages 245-245
Overview of Applications Formats....Pages 247-252
Blot Formats: Nucleic Acids....Pages 253-296
Blot Formats: Proteins and Glycoproteins....Pages 297-303
Front Matter....Pages 245-245
In Situ Formats....Pages 304-392
Quantitative Formats....Pages 393-427
Back Matter....Pages 429-436