Cell Cycle — Materials and Methods

Front Matter....Pages I-XXX
Front Matter....Pages 1-1
Use of Immunohistochemistry in Cell Cycle Studies....Pages 3-14
Immunofluorescence Techniques in Cell Cycle Studies....Pages 15-28
Fluorescence in Situ Hybridization and Human Cell Cycle Genes....Pages 29-45
Techniques for Monitoring Cell Cycle Phases....Pages 46-52
Centrifugal Elutriation....Pages 53-62
Synchronization of Mammalian Cells....Pages 63-74
Microinjection and Electroporation of Macromolecules into Live Cells....Pages 75-86
Stable and Transient Transfections in Cell Cycle Studies....Pages 87-92
Quantification of Premature and Normal Mitosis....Pages 93-99
Front Matter....Pages 101-101
Preparation of Xenopus Egg Extracts and Their Utilization in Cell Cycle Studies....Pages 103-116
Front Matter....Pages 117-117
Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe ....Pages 119-131
Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces pombe ....Pages 132-142
Front Matter....Pages 143-143
Identification of Novel Cell Cycle Targets Using Small Molecule Ligands....Pages 145-156
Bacterial Expression and in Vitro Assay of Cell Cycle Kinases....Pages 157-168
Heterologous Expression and Secretion of Cell Cycle Proteins Using the Yeast Picchia pastoris ....Pages 169-174
SV40-Based in Vitro DNA Replication Assay....Pages 175-185
From Sequence to Function — Structural Motifs of Cell Cycle Proteins....Pages 186-199
Purification of Cell Cycle Kinases....Pages 200-210
Interaction-Based Cloning of Novel Proteins....Pages 211-230
Expression of Cell Cycle Proteins Using Recombinant Baculoviruses....Pages 231-242
Front Matter....Pages 143-143
Cell Cycle-Regulated Transcription Factors; Bandshift Assays....Pages 243-249
Immunoprecipitation and Immunoblotting in Cell Cycle Studies....Pages 250-263
Protein Mapping in Cell Cycle Studies....Pages 264-270
From Peptide to Purified-Antibody....Pages 271-280
Back Matter....Pages 281-286