Microinjection and Transgenesis: Strategies and Protocols

Front Matter....Pages I-XIII
Front Matter....Pages 1-1
Protocols for the Manual and Automatic Microinjection of Somatic Cells in Culture and for the Analysis of Microinjected Cells....Pages 3-30
Automated Computer-Assisted Microinjection into cultured somatic cells....Pages 31-46
Immunofluorescence and Immunoelectron Microscopy of Microinjected and Transfected Cultured Cells....Pages 47-79
Videomicroscopy of Living Microinjected Hippocampal Neurons in Culture....Pages 81-94
Preparation, Purification, and Analysis of Synthetic Oligonucleotides Suitable for Microinjection Experiments....Pages 95-112
Colorimetric in Situ Hybridization....Pages 113-129
Manual Microinjection: Measuring the Inhibitory Effect of HIV-1-Directed Hammerhead Ribozymes in Tissue Culture Cells....Pages 131-143
Cellular Microbiochemistry....Pages 145-154
A New Strategy for Studying Microtubule Transport and Assembly During Axon Growth....Pages 155-170
The Use of Microinjection to Study Signal Transduction in Mammalian Cells....Pages 171-183
Front Matter....Pages 185-185
Supply and Husbandry of Mice for Transgenic Science....Pages 187-215
Mouse Male Germ Cells in Culture: Toward a New Approach in Transgenesis?....Pages 217-227
Sperm-Mediated Gene Transfer....Pages 229-254
Application of Ligand-Dependent Site-Specific Recombination in ES Cells....Pages 255-274
A Modified Protocol for the Generation of Transgenic Mice and Freezing of Mouse Embryos....Pages 275-284
The Use of Morula Aggregation to Generate Germline Chimeras from Genetically Modified Embryonic Stem Cells....Pages 285-296
The Generation of Transgenic Mice with Yeast Artificial Chromosomes....Pages 297-308
Following Up Microinjected mRNAs: Analysis of mRNA Poly(A) Tail Length During Mouse Oocyte Maturation....Pages 309-322
Mouse Preimplantation Embryos and Oocytes as an In Vivo System to Study Transcriptional Enhancers....Pages 323-349
The Use of Mouse Preimplantation Embryos for the Identification of DNA-Binding Proteins Essential in Early Development....Pages 351-367
Front Matter....Pages 185-185
Application of Transgenes and Transgenic Mice to Study Gene Activity from the Oocyte to the Early Embryo....Pages 369-412
Transitory Transgenic Analysis as an In Vivo System to Study Promoter Regulatory Elements....Pages 413-425
Transgenic Analysis of Embryonic Gene Expression Using LacZ as a Reporter....Pages 427-437
Autonomous Cell Labeling Using a laacZ Reporter Transgene to Produce Genetic Mosaics During Development....Pages 439-458
Multiple Developmental Functions of the Zinc Finger Gene Krox-20 Are Revealed by Targeted Insertion of the E. coli lacZ Coding Sequence....Pages 459-476
Transgenic Mouse Strategies in Virus Research....Pages 477-504
Isolation of Mutated Genes from Transgene Insertion Sites....Pages 505-520
Contributions of Transgenic and Knockout Mice to Immunological Knowledge....Pages 521-559
Generation and Application of Transgenic Rabbits....Pages 561-586
Microinjection of Drosophila Eggs....Pages 587-603
Back Matter....Pages 605-619