Animal Cell Technology: From Target to Market: Proceedings of the 17th ESACT Meeting Tylosand, Sweden, June 10–14, 2001

Front Matter....Pages i-lxxv
Front Matter....Pages 1-1
In Vitro Testing of a New Substance with Anti-Tumor Activity on Mammalian Cells Using Flow Cytometry....Pages 3-5
Destabilization of Green Fluorescent Protein by Substitution of Its Amino-Terminal Residue....Pages 6-9
Anti-Tumor Activity of Noguchi Catalyser 21 ™ , a Mineral Water Containing Natural Leaf Soil....Pages 10-12
Fermented Milk, Kefram-Kefir Enhances Glucose Uptake into Insulin-Responsive Muscle Cells....Pages 13-15
Optimization of Medium for M2 (Anti-Flag) Hybridoma Using Factorial Matrix Designs....Pages 16-19
Neurite Outgrowth Promoting Substance from a Marine Alga, Sargassum Macrocarpum ....Pages 20-22
Neutralization of the Biological Activity of Glycosylated and Non-Glycosylated hGM-CSF by Monoclonal Antibodies....Pages 23-25
High Cell Density Cultivation of Mast Cells in Fluidized-Bed and Fixed-Bed Bioreactors....Pages 26-28
Front Matter....Pages 29-29
Over-Expression of Protein Kinase Bα Enhances Recombinant Protein Expression in Transient Systems....Pages 31-36
100 Liter Transient Transfection....Pages 37-44
Application of Alphavirus Vectors in Drug Discovery....Pages 45-51
The Use of UCOE Vectors in Combination with a Preadapted Serum Free, Suspension Cell Line Allows for Rapid Production of Large Quantities of Protein....Pages 52-57
Overexpression of a Transcription Factor to Maximize Glycoprotein Production in an Inducible Expression System....Pages 58-65
Glucose Dehydrogenase as Detector Protein of Recombinant Fusion-Proteins Directly in SDS Gels....Pages 66-68
Successful Transfection of Partially Purified DNA in the HEK293EBNA Cell Line for Transient Expression....Pages 69-71
Capillary-Aided Cell Cloning....Pages 72-74
The Comparative Study of Cultural and Productive Characteristics of Different Recombinant Cell Lines Producing Human Erythropoietin....Pages 75-78
The Use of a C-FOS -GFP Reporter System for Monitoring Apoptosis of Animal Cells Induced by Exotoxins of Pseudomonas Aeruginosa ....Pages 79-82
High Performance Transient Expression in Mammalian Cells....Pages 83-87
In 30 Days from Plasmid to Product: Expression of a Secreted Fusion Protein Using Hek293-EBNA Cells....Pages 88-90
Front Matter....Pages 29-29
SF9 Cells Infection and Fuc-TIII Quality in Serum-Free and Serum-Containing Media....Pages 91-93
Rapid Expression of Recombinant Proteins in Modified CHO Cells Using the Baculovirus System....Pages 94-100
Recombinant Glycoprotein Production by CHO Cells in Low Protein Serum Free Medium....Pages 101-106
Arginine Is a Limiting Essential Amino Acid in Batch Cultures of Recombinant Chinese Hamster Orvary Cells Producing Thrombopoietin....Pages 107-109
Production of Recombinant Allergen Proder P 1 by CHO Cells Adapted to Grow in Serum-Free Suspension....Pages 110-113
Pilot Scale Production and Purification of a Soluble E-Selectin-IgG Chimeric Protein....Pages 114-117
Front Matter....Pages 119-119
Environmental Effects on Cell Physiology and Metabolism: Response to Elevated pC0 2 ....Pages 121-128
Improved Dual-Regulated Expression Systems for Independent Control of Two Different Transgenes....Pages 129-135
Metabolic Flux Analysis in Mammalian Cells — Network Modelling as an Example for Metabolic Design in Recombinant BHK Cells....Pages 136-142
Assessment of Keratin Expression and Xenobiotic Metabolism in Cultured Normal and Transformed Human Oral Keratinocytes....Pages 143-148
Regulation of Cell Cycle and Productivity in NS0 Cells by the Over-Expression of p21 CIP1 ....Pages 149-155
Loss of Productivity of a Transfectoma in Long-Term Culture: Recovery of Expression....Pages 156-159
Adaptation and Selection of NSO Myeloma Cell Lines Producing Recombinant Monoclonal Antibodies in Protein-Free Medium....Pages 160-163
Specific Effects of Synthetic Oligopeptides in Animal Cell Culture....Pages 164-167
Differential Enhancement of Monoclonal Antibody Production by Retinoids in Human Hybridomas....Pages 168-170
Regulation Mechanisms of Antibody Production from Human PBMC Immunized in vitro ....Pages 171-174
Influences of Glutamine Concentration on Cellular Metabolism in Continuous Culture of Hybridoma....Pages 175-178
Study of NS0 Cell Line Metabolism in Lipid Supplemented Protein Free Media....Pages 179-182
Metabolic Shifts in Hybridoma Cells Utilising Wheat Peptides....Pages 183-184
Bi-Phasic Culture Strategies Based on Medium Formulation: Substitution of Glucose by Galactose in CHO Culture....Pages 185-188
Front Matter....Pages 119-119
New Advanced Protein-Free, Animal Component-Free Medium for Recombinant Protein Expression in Adherent CHO Cell Cultures....Pages 189-192
Na + Is Responsible for Intra-Run Variations of Specific Productivity of CHO Cell Lines Producing Human Anti Rh D IgG in Bioreactors....Pages 193-196
The Influence of pH on Cell Growth and Specific Productivity of Two CHO Cell Lines Producing Human Anti Rh D IgG....Pages 197-199
The Modification of a Serum-Free Media Formulation for the Production of Reovirus and the Growth of Vero, MRC-5, MDCK and BHK Cell Lines....Pages 200-203
The Role of Vitamins in Cell Culture Media....Pages 204-206
Effect of Sodium Butyrate on Glycosylation of Recombinant Erythropoietin....Pages 207-209
Identification of Betacellulin as a Major Growth Factor in Foetal Bovine Serum and Development of a Recombinant Betacellulin Analogue for Use in Serum-Free Cell Culture....Pages 210-212
Monitoring Growth and Death of Vero Cells Cultivated in Bioreactor with Serum-Containing and Serum-Free Media....Pages 213-216
Effects of a Rice Protein Hydrolysate on Growth of CHO Cells and Production of Human Interferon-γ in a Serum-Free Medium....Pages 217-219
Suppressive Effect of Electrolyzed-Reduced Water on the Growth of Cancer Cells and Microorganisms....Pages 220-223
Transient Bcl- x L Overexpression Has Different Effects on Protein Production in Various CHO-Derived Cell Lines....Pages 224-226
Proteolytic Potential during Batch Cultivation in Serum Free Media of an IFN-γ Producing CHO Cell Line....Pages 227-229
Genetic Strategies for Apoptosis Protection of Hybridoma Cells Based on Overexpression of Cellular and Viral Proteins....Pages 230-233
Modulation of Cell Proliferation by Metabolic Engineering....Pages 234-237
S-Phase Synchronized CHO Cells Show Elevated Transfection Efficiency and Expression Using CA/PO 4 ....Pages 238-240
Expression of Yeast Pyruvate Carboxylate in hGM-CSF-Producing CHO Cells....Pages 241-243
The Influence of Culture Conditions on the Development of Trans-Epithelial Electrical Resistance (TEER) and the Junctional Complex of MDCK Cells....Pages 244-246
Bioreactor for Pharmaceutical Screening Generally Applicable for Primary Cells....Pages 247-249
Increasing Population of Amniotic Epithelial Cells....Pages 250-252
Evaluation of the Biological Activity of Some Hormones, Growth Factors and Drugs on Cultured Cells, Isolated from Animal and Human Reproductive Organs....Pages 253-257
Front Matter....Pages 119-119
Cultivation of Human Articular Chondrocytes for Autologous Transplantation....Pages 258-260
A Cell Culture System from the Eye Stalk of Penaeus Indicus ....Pages 261-265
Cytotoxicity of Pyrethroid Insecticides in Rat Hepatoma-Derived Fa32 Cells....Pages 266-268
New Technologies for Automated Cell Counting Based on Optical Image Analysis “The Cellscreen”....Pages 269-273
Application of an Acceleration-Stat to Study Insect Cell Metabolism....Pages 274-276
Front Matter....Pages 277-277
Factorial Design and Analysis for Rapid Development of an Antibody Production Process in CHO Cells....Pages 279-286
Development of a Large Scale Process for the Production of Recombinant Truncated Factor VIII in CHO Cells under Cell Growth Arrest Conditions....Pages 287-292
Continuous Perfusion versus Discontinuous Fed-Batch....Pages 293-300
The Use of Hydrocyclones for Mammalian Cell Retention in Perfusion Bioreactors....Pages 301-306
High Throughput Cultivation of Animal Cells Using Shaken Microplate Techniques....Pages 307-312
Continuous Isolation of rFVIII from Mammalian Cell Culture....Pages 313-317
Sodium Butyrate Treatment: A Study of Critical Parameters Using CHO Cell Lines Expressing the Green Fluorescent Protein....Pages 318-324
Development of a Robust Protocol for the Production of Recombinant Proteins up to 20-L Scale Using BEVS....Pages 325-327
Optimising the Production of a Nuclear Receptor in SF9 Cells Using Response Surface Analysis....Pages 328-331
Growth of High-Five ™ Cells on Cultispher-S Microbarriers....Pages 332-334
Catching the Wave: The BEVS and the Biowave....Pages 335-337
Physiology of Insect Cells Cultured in a New Serum-Free Medium....Pages 338-340
Characterisation and Evaluation of a New Screening System for Animal Cell Culture....Pages 341-343
Development of Bioprocess Concepts on Vaccine Production: Influenza Virus as an Example....Pages 344-346
Assay of Serum and Animal Protein Free Media for the Production of a Veterinary Rabies Vaccine by BHK-21 Cells....Pages 347-350
Front Matter....Pages 277-277
Cell Cultivation System on the Basis of Porous Ceramic for the Production of Virus-Like Particles....Pages 351-353
Influence of Cell Culture Media on Adhesion of Animal Cells on Microcarriers....Pages 354-357
Comparison of Three Different Serum Free Media: Scale up of a Semi-Continuous CHO Cell Process in an Industrial Application....Pages 358-361
Use of Plant Peptone-Containing Serum-Free Media for the Cultivation of CHO Cells in Suspension and on Microcarriers....Pages 362-364
Application of Protein-Free Cell Culture Media for the Manufacturing of Biopharmaceuticals....Pages 365-368
Fluid Bed Granulation as an Alternative to Milled Powders to Improve Performance of Dry-Form, Serum-Free Nutrient Media....Pages 369-371
NanoColloids: A New Method for Solubilization of Lipophilic Substances Used in Cell Culture....Pages 372-374
Development and Validation of a Novel Process for the Production of Powdered Cell Culture Media....Pages 375-378
Separation of Mammalian Cells Using a Specially Designed Rotating Disc Filter....Pages 379-381
Comparison of Fluidised Bed and Ultrasonic Cell-Retention Systems for High Cell Density Mammalian Cell Culture....Pages 382-385
Ultrasonic Cell Separation — Production of Monoclonal Antibodies in Continuous Perfusion Cultures....Pages 386-388
Increased Production Yields of Native Interferon-γ in Membrane Bioreactors by Continuous Harvest....Pages 389-392
Production of rhEPO in Hollow Fibers’ Bioreactor....Pages 393-395
Production of rhEPO under Variants of Cultivation Media....Pages 396-398
Solution to the high Dissolved CO 2 Problem in High-Density Perfusion Culture of Mammalian Cells....Pages 399-402
Perfusion Cell Culture in Disposable Bioreactors....Pages 403-409
Process Development in a Packed Bed Bioreactor....Pages 410-411
The Art & Science of Micro-Sparging in High-Density Perfusion Cultures of Animal Cells....Pages 412-415
Culture of the CB.Hep-1 Hybridoma Using Different in vitro Systems....Pages 416-418
Selection of Suitable Anti-rHBsAg Mabs for in vitro Production....Pages 419-421
Front Matter....Pages 277-277
Reorganizing of Monoclonal Antibodies Process in the Era of Human Genome Cloning....Pages 422-426
Production of a Highly Pure Mab from Cell-Pharm System CP-2500....Pages 427-430
How Does Protein Free Medium Turbodoma™ HP-I Perform in Mouse Hybridoma Cultures in Different Culture Systems?....Pages 431-433
Effect of Seed Cell Density on Specific Growth Rate Using CHO Cells as Model....Pages 434-437
Adaptation and Growth Kinetics of the Universal Host CHO Cell Lines in Serum-Free Medium....Pages 438-440
Process Development for Functional Membrane Receptor Production in Mammalian Cells....Pages 441-445
Development of an Automated, Adaptive Model-Based Control for the Effective Cultivation of Hybridoma Cells....Pages 446-448
The One Step Inoculation Concept: A New Seed-Train Expansion for Recombinant Mammalian Cell Lines....Pages 449-451
In-Situ Microscopy for On-Line and In-Line Monitoring of Cell Populations in Bioreactors....Pages 452-454
Measurement of the Viable Cell Density: Validation and Integration of On-Line and Off-Line Capacitance Biomass Monitors for Cell Culture Processes....Pages 455-458
Robot Automation of Sampling and Sample Management during Cultivation of Mammalian Cells in Pilot Scale....Pages 459-462
Rapid Estimation of Human Monoclonal Antibody (IgG4) Concentration in Cell Culture Supernatants....Pages 463-465
Use of Ion-Exchange Membrane Adsorbers for Production of Biopharmaceuticals....Pages 466-468
Front Matter....Pages 469-469
Pharmaceuticals Produced by Biotechnology: Quality and Safety Issues....Pages 471-476
Factory IQ/OQ, A New Approach for Streamlining Validation of Cell Culture Equipment....Pages 477-481
Critical Issues for the Use of Tangential Flow Filtration Systems for Cell Harvesting Applications....Pages 482-484
A General Method for the On-Line RT-PCR Detection of Retrovirus Contamination in Cell Lines Used for Protein and Viral Vector Production....Pages 485-487
Novel Assay for Protein Impurities in Biopharmaceuticals Based on Fluorescence Intensity Distribution Analysis (FIDA)....Pages 488-490
Development of Mammalian Cell Bioreactor Processes....Pages 491-493
Front Matter....Pages 495-495
Development of Microgranulated Form of the Live Measles Vaccine....Pages 497-499
Front Matter....Pages 501-501
Bioprocess Development for the Cultivation of Human T-Lymphocytes....Pages 503-509
Adenovirally Modified Dendritic Cells for Immunotherapy: From Basic Development to Clinical Application....Pages 510-516
GMP Compliant Expansion of Hematopoietic Stem Cells (CD34+) Selected from Cord Blood for a Phase I Cell Therapy Trial....Pages 517-522
Multi-Plasmid Vaccines for HIV-1: Issues and Advancements....Pages 523-531
Continuous Retroviral Vector Production in a Perfusion Bioreactor System....Pages 532-534
Monolayer and Suspension Cultures of HEK 293 Cells for Recombinant Adenovirus Production for Gene Therapy: Effect of “Cell Passage Number”....Pages 535-537
Possible Strategies for the Production of Viral Vector: The Role of Engineering Design....Pages 538-540
Establishing Novel Hepatoma Cell Line for Bioartificial Liver....Pages 541-544
An Improved Oxygenation Hollow Fiber Bioreactor for the Cultivation of Liver Cells....Pages 545-547
Medium for Gene Therapy: Improved Protein-Free Media for Growth and Production of Viral Vectors for Use in Gene Therapy....Pages 548-552
Induction of a T Helper Cell Response against the Tumor Associated Antigen Her-2 Using Monocyte-Derived Dendritic Cells....Pages 553-557
Cultivation of Human HCMV Specific Lymphocytes — An Example for Adoptive Immunotherapy....Pages 558-561
Application of hTERT Promoter to Cancer Therapy....Pages 562-564
Establishment of a Genetic System to Coordinate Feeder Cell Growth in Cocultures with Primary Cells....Pages 565-567
Construction and Operation of a Bioreactor for Three-Dimensional Cartilage-Implants....Pages 568-570
Tissue Engineering of Meniscal Cartilage....Pages 571-573
Anti-Oxidative Water Improves Diabetes....Pages 574-577
Effect of Transfection-Induced Changes in Expression of Insulin-Like Growth Factor Binding Proteins on Secretory Activity and Responses of Ovarian Cell Cultures....Pages 578-580
Cultivation of Primary Osteogenic Cells in Serum-Reduced or Serum-Free Culture Media: Attachment, Proliferation and Differentiation....Pages 581-583
Back Matter....Pages 585-595