Experimental Glycoscience: Glycochemistry

Front Matter....Pages I-XIII
Front Matter....Pages 1-1
Chemical Liberation of N -linked Oligosaccharides from Glycoproteins....Pages 5-6
Release of N -glycans by Enzymatic Methods....Pages 7-11
Release of O -glycans by Chemical Methods....Pages 12-13
Release of O -glycans by Enzymatic Methods....Pages 14-17
Ceramidase and Related Enzymes....Pages 18-21
Labeling of Oligosaccharides....Pages 22-23
Chemical Labeling of Sialyloligo/polymer....Pages 24-27
Separation of Oligosaccharides by 2D HPLC....Pages 28-29
Analysis of Oligosaccharides by Capillary Electrophoresis....Pages 30-34
Energy-Resolved Mass Spectrometry (ERMS) of Oligosaccharide....Pages 37-38
LC/MS of N -Linked Oligosaccharides....Pages 39-41
Convenient Structural Characterization of Intact Glycosphingolipids by MALDI-TOF Mass Spectrometry with Increased Laser Power and Cooling Gas Flow....Pages 42-44
Structural Analyses of Glycoconjugates by NMR....Pages 45-50
Sugar Chain Analysis by Enzymatic Digestion and 2D Mapping by HPLC....Pages 51-53
Analysis of Binding Sites of Sugar Chains by Methylation Analysis....Pages 54-55
Glycan Profiling....Pages 56-59
Monoclonal Antibody as a Clue to Structural Analysis of Bioactive Functional Glycoconjugates....Pages 60-63
Microsequencing of Functional Chondroitin Sulfate Oligosaccharides....Pages 64-69
Structural Characterization of PA-oligosaccharide Isomers Derived from Glycosphingolipids by MALDI-TOF Mass Spectrometry....Pages 70-73
Structural Analysis of Phospho-Glycosphingolipids in Lower Animals....Pages 74-76
Front Matter....Pages 1-1
Structural Analysis of Polysialic Acid....Pages 77-81
Milk Oligosaccharides: Structural Characterization and Future Aspects....Pages 82-86
An XML Description of Carbohydrate Structures....Pages 89-91
Construction of a Diagnostic Library for Glycans Using Multistage Tandem Mass Spectrometry (MS n )....Pages 92-93
A Method for Large-Scale Analysis for N -linked Glycoproteins by the Glycosylation Site-Specific Stable Isotope-Labeling and LC/MS Shotgun Technology....Pages 94-97
Mass Spectrometry of Glycopeptides....Pages 98-99
Development of MS Method for the Analysis of Sugar Peptide....Pages 100-104
Calculations for Saccharides by the Use of MS Data....Pages 105-106
Molecular Modeling of Oligosugar Structures....Pages 109-110
Prediction of Sugar-Binding Sites on Proteins....Pages 111-114
Frontal Affinity Chromatography: An Effective Analytical Tool for Protein-Sugar Interaction....Pages 117-120
Analyses of Sugar-Protein Interactions by NMR....Pages 121-123
Surface Plasmon Resonance and Sugar Chip Analysis for Sugar Chain-Protein Interactions....Pages 124-126
Analysis of Interactions Between Carbohydrates and Proteins Using Capillary Affinity Electrophoresis....Pages 127-131
Equilibrium Dialysis....Pages 132-133
Interaction Assay of Oligosaccharide with Protein Using Fluorescence Polarization (FP) and Fluorescence Correlation Spectroscopy (FCS)....Pages 134-137
Development of Neoglycoconjugate Probes and Detection of Lectins....Pages 138-141
Front Matter....Pages 143-143
Synthesis of Glycolipid and Its Application....Pages 147-150
Chemo-Enzymatic Synthesis of Glycoconjugates....Pages 151-154
Construction of O -Linked Glycopeptide Library Using Human Glycosyltransferases....Pages 155-157
Front Matter....Pages 143-143
Alteration of N -Linked Oligosaccharides....Pages 158-163
Stationary Solid-Phase Reaction (SSPR) for Oligosaccharide Synthesis....Pages 164-165
Sugar Chain Synthesis by the Use of Cell Functions....Pages 166-168
Glyco-Chemistry Cycle System Based on Glycosidases....Pages 169-172
Endoglycosidases (Glycosaminoglycans)....Pages 173-176
Oligosaccharide Synthesis Based on Combinatorial Chemistry and Labo Automation....Pages 177-181
Endoglycosidases (Glycoproteins)....Pages 182-185
Glycosaminoglycans....Pages 186-190
Recent Advances in the Production of Mammalian-Type Sugar Chains in Yeast....Pages 191-194
Solid-Phase Synthesis of Glycopeptides....Pages 195-199
Efficient Synthesis of Oligosaccharides and Synthesis of Pathogen-Associated Molecular Patterns for Their Biofunctional Studies....Pages 200-205
Sugar Polymers (Dendrimers and Pendant-Type Linear Polymers)....Pages 206-209
Rapid Synthesis of Oligosaccharides: Resin Capture-Release Strategy....Pages 210-216
Back Matter....Pages 217-251